Imaris Stitcher Technology
Stitching tiles into one large image requires large image handling as well as alignment precision for a high quality result. Imaris Stitcher's algorithm addresses the imperfections in microscope stage positioning including camera rotation relative to the stage to provide the best alignment results across the entire fused image.
- 校准和缝合的2 d、3 dor 4D overlapping image blocks
- Alignment and stitching in XYZ
- Possible alignment and stitching of multiple tiles with many channels*
- Aligns and saves images up to Terabytes in size**
- Alignment based on the image content in the overlapping regions
- Corrects for camera vs microscope stage rotation. This systematic error of the acquisition can be estimated from just a few pairs of overlapping images which contain content and then used to reposition all images even if the signal is insufficient.
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*Visualization requires at least 1MB VRAM per image, per channel; Opening 500 images with 3 channels requires at least 1500MB graphics memory.
**Minimum RAM requirements: around ten times the size of the largest volume overlap region.