Learning More About DNA G-Wires

High-resolution AFM imaging in ionic aqueous solution gave detailed insight into the structure and formation of DNA G-wires. The observed nanoscale periodic features allowed researchers to classify the G-wires into different species.

G-Wire DNA是通过短DNA片段的自组装形成的核酸结构系列。这些类似的电线结构不仅因为它们而引起了兴趣biological implicationsbut also for potential use in nanoelectronic and nanosensor devices. However, the details of how G-wires form and thebiomolecular structuresthat result are still not clearly understood.

为此，新加坡南洋技术大学的研究人员调查了短件的自我汇编Tetrahymena端粒DNA序列D [g₄T₂G₄]. They acquired high-resolution AFM images in physiologically relevant conditions and observed a variety of wire-like structures with periodic features as small as 0.9 nm.

From this data, the researchers classified G-wires into different species according to height, periodicity, and handedness. Molecular modeling and simulations also allowed them to identify a specific molecular structure (rotamer) that could explain the experimental observations.

通过丰富对G线形成的理解，结果可能对与DNA自组装有关的一系列生物和纳米技术应用来证明有价值。万博电脑网页版登录

使用仪器

Cypher es.withHeating and Cooling Sample Stage

使用的技术

浸入磷酸钾缓冲溶液中的G线被成像Cypher es.AFM in.tapping mode。Experiments were performed with the solution cooled to a constant 10 °C using theHeating and Cooling Sample Stage。由于CyPher AFMS常规实现的无与伦比的空间分辨率，清楚地看到了纳米级结构。此外，Bluedrivetechnology helped make tapping in liquid simpler and more stable. The G-wires’ nanomechanical properties, including stiffness and loss tangent, were characterized withAM-FM Viscoelastic Mapping Mode(see supplementary information available at the article link below).

引文：K. Bose，C.J.Lech，B.Heddi和A.T.Phan，水溶液中DNA G线的高分辨率AFM结构。Nat. Commun.9, 1959 (2018).https://doi.org/10.1038/s41467-018-04016-y

Note:The original article featured above was published as Open Access under a Creative Commons license. The data shown here are reused under fair use of the original article, which can be accessed through the article link above.